WB
Various lysates were subjected to SDS PAGE followed by western blot with ROR1 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody as loading control.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using antibody at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | ROR1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ROR1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:6000 IHC:1:300-1:1200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ROR1 |
|---|---|
| Gene Synonyms | NTRKR1 dJ537F10.1 |
| Gene Full Name | receptor tyrosine kinase like orphan receptor 1 |
| Gene Summary | This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012] |
| Molecular Weight(MW) | 130 kDa |
| Cellular Localization | Cell projection, axon |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ROR1 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody as loading control.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using antibody at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:6000 IHC:1:300-1:1200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643827