WB
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human ANGPT1 with DDK (FLAG) tag probed with ANGPT1 antibody (1ug/ml) in Lane A and probed with anti-DDK Tag (1:1000,in Lane C. Mock-transfected HEK293 probed with ANGPT1 antibody (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence..