WB
Various lysates were subjected to SDS PAGE followed by western blot with VARS2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using VARS2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VARS2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).IP
IP result of anti-VARS2 (IP:4ug; Detection: 1:500) with HeLa cells lysate 2.4mg.| Product Name | VARS2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human VARS2 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate. |
|
| Species Reactivity | Human |
| Concentration | 0.3mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VARS2 |
|---|---|
| Gene Synonyms | VALRS VARSL VARS2L COXPD20 |
| Gene Full Name | valyl-tRNA synthetase 2, mitochondrial |
| Gene Summary | This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
| Molecular Weight(MW) | 118kDa |
| Cellular Localization | Mitochondrion. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with VARS2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using VARS2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VARS2 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).
IP
IP result of anti-VARS2 (IP:4ug; Detection: 1:500) with HeLa cells lysate 2.4mg.| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM644175