WB
Western blot analysis of various lysates using NSD3/WHSC1L1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.WB
Western blot analysis of lysates from wild type (WT) and NSD3/WHSC1L1 knockout (KO) 293T cells, using NSD3/WHSC1L1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.| Product Name | NSD3/WHSC1L1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 400-600 of human NSD3/WHSC1L1 (NP_060248.2). |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NSD3 |
|---|---|
| Gene Synonyms | KMT3F KMT3G WHISTLE WHSC1L1 pp14328 |
| Gene Full Name | nuclear receptor binding SET domain protein 3 |
| Gene Summary | This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015] |
| Molecular Weight(MW) | 162kDa(Observed MW 180kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates using NSD3/WHSC1L1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
WB
Western blot analysis of lysates from wild type (WT) and NSD3/WHSC1L1 knockout (KO) 293T cells, using NSD3/WHSC1L1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.| Application Notes | WB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Data sheet for OM644244