Application
TRIM72 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TRIM72 antibody detected the TRIM72 protein (arrow).Application
TRIM72 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM72 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | TRIM72 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM72; MG53; Tripartite motif-containing protein 72; Mitsugumin-53 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MG53 |
|---|---|
| Alternative Names | TRIM72 MG53 Tripartite motif-containing protein 72 Mitsugumin-53 |
| Molecular Weight(MW) | 52731 Da |
| Function | Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity) |
| Tissue Specificity | This TRIM72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-335 amino acids from the C-terminal region of human TRIM72. |
| Cellular Localization | Cell membrane, sarcolemma (By similarity). Cytoplasmic vesicle membrane (By similarity). Note=Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine (By similarity) |
| Entrez Gene | 493829 |
|---|---|
| Nucleotide Accession | NP_001008275.2 |
Application
TRIM72 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TRIM72 antibody detected the TRIM72 protein (arrow).
Application
TRIM72 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM72 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM223663