WB
Various lysates were subjected to SDS PAGE followed by western blot with TRIM72 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).IP
IP result of anti-TRIM72 (IP:4ug; Detection: 1:300) with mouse heart tissue lysate 4mg.| Product Name | TRIM72 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TRIM72 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIPWB |
| WB:1:2000-1:10000 IF-P:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.8mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM72 |
|---|---|
| Gene Synonyms | MG53 |
| Gene Full Name | tripartite motif containing 72 |
| Gene Summary | Enables identical protein binding activity. Predicted to be involved in several processes, including plasma membrane repair; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein homooligomerization. Predicted to act upstream of or within negative regulation of insulin receptor signaling pathway; negative regulation of insulin-like growth factor receptor signaling pathway; and negative regulation of myotube differentiation. Predicted to be located in cytoplasmic vesicle membrane. Predicted to be active in cytoplasm and sarcolemma. [provided by Alliance of Genome Resources, Feb 2025] |
| Molecular Weight(MW) | 53kDa |
| Cellular Localization | Cell membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with TRIM72 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IP
IP result of anti-TRIM72 (IP:4ug; Detection: 1:300) with mouse heart tissue lysate 4mg.| Application Notes | WB:1:2000-1:10000 IF-P:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643978