Application
Western Blot at 1:2000 dilution Lane 1: HCT116 whole cell lysate Lane 2: human skeletal muscle lysate Lane 3: mouse skeletal muscle lysate Lane 4: rat skeletal muscle lysate Lysates/proteins at 20 ug per lane.Application
TRIM72 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | TRIM72 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tripartite motif-containing protein 72, Mitsugumin-53, Mg53, TRIM72 ( HGNC:32671 ), MG53 |
| Product description | TRIM72 is a muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).1) Park, E.Y., et al. Proteins 78(3):790-795(2010) |
| Immunogen | This TRIM72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-327 amino acids from the C-terminal region of human TRIM72. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM72 |
|---|---|
| Alternative Names | Tripartite motif-containing protein 72 Mitsugumin-53 Mg53 TRIM72 ( HGNC:32671 ) MG53 |
| Molecular Weight(MW) | 53 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: HCT116 whole cell lysate Lane 2: human skeletal muscle lysate Lane 3: mouse skeletal muscle lysate Lane 4: rat skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Application
TRIM72 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM294111