Application
UBE3A Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the UBE3A antibody detected the UBE3A protein (arrow).Application
UBE3A Antibody (N-term) (AP14799a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE3A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | UBE3A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE3A; E6AP; EPVE6AP; HPVE6A; Ubiquitin-protein ligase E3A; E6AP ubiquitin-protein ligase; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | E6AP EPVE6AP HPVE6A |
|---|---|
| Alternative Names | UBE3A E6AP EPVE6AP HPVE6A Ubiquitin-protein ligase E3A E6AP ubiquitin-protein ligase Human papillomavirus E6-associated protein Oncogenic protein-associated protein E6-AP Renal carcinoma antigen NY-REN-54 |
| Molecular Weight(MW) | 100688 Da |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo |
| Tissue Specificity | This UBE3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685-715 amino acids from the N-terminal region of human UBE3A. |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 7337 |
|---|---|
| Nucleotide Accession | NP_570853.1 NP_000453.2 NP_570854.1 |
Application
UBE3A Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the UBE3A antibody detected the UBE3A protein (arrow).
Application
UBE3A Antibody (N-term) (AP14799a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE3A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM224111