Application
The anti-PRKAB1 Pab is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).Application
Western blot analysis of anti-PRKAB1 Antibody (N-term) in mouse heart lysates (35ug/lane). PRKAB1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | AMPK beta (PRKAB1) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAB1; AMPK; 5'-AMP-activated protein kinase subunit beta-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AMPK |
|---|---|
| Alternative Names | PRKAB1 AMPK 5'-AMP-activated protein kinase subunit beta-1 |
| Molecular Weight(MW) | 30382 Da |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) |
| Tissue Specificity | This PRKAB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human PRKAB1. |
Application
The anti-PRKAB1 Pab is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).
Application
Western blot analysis of anti-PRKAB1 Antibody (N-term) in mouse heart lysates (35ug/lane). PRKAB1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM226414