Application
A. Western blot analysis on human brain tissue lysate using anti-Phospho-AMPK beta 1(pS181) RabMAb (Cat. #AJ1041a), 1:20,000 dilution. Cells were either (A) untreated (B) treated with Alkaline Phosphatase.Application
B. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-AMPK RabMAb(Cat. #AJ1041a).Application
C. Immunofluorescent staining of HeLa cells using anti-AMPK RabMAb (Cat. #AJ1041a).| Product Name | AMPK-beta1 Antibody Phospho-pS181 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAB1, AMPK, 5'-AMP-activated protein kinase subunit beta-1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2181Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AMPK |
|---|---|
| Alternative Names | PRKAB1 AMPK 5'-AMP-activated protein kinase subunit beta-1 |
| Molecular Weight(MW) | 30382 Da |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta was used as immunogen. |
| Entrez Gene | 5564 |
|---|
Application
A. Western blot analysis on human brain tissue lysate using anti-Phospho-AMPK beta 1(pS181) RabMAb (Cat. #AJ1041a), 1:20,000 dilution. Cells were either (A) untreated (B) treated with Alkaline Phosphatase.
Application
B. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-AMPK RabMAb(Cat. #AJ1041a).
Application
C. Immunofluorescent staining of HeLa cells using anti-AMPK RabMAb (Cat. #AJ1041a).| Application Notes | WB:1:20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM226424