Application
A. Western blot analysis of anti-AMPK beta-1 RabMAb (Cat. #AJ1038a), dilution 1:5,000. A-NIH 3T3 B: Hela C: A431 D: PC12Application
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-AMPK beta-1 RabMAb (Cat. #AJ1038a).| Product Name | AMPK beta I Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAB1, AMPK, 5'-AMP-activated protein kinase subunit beta-1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y367 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AMPK |
|---|---|
| Alternative Names | PRKAB1 AMPK 5'-AMP-activated protein kinase subunit beta-1 |
| Molecular Weight(MW) | 30382 Da |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) |
| Tissue Specificity | A synthetic peptide corresponding to residues in the human AMPK beta-1 subunit was used as an immunogen. |
| Entrez Gene | 5564 |
|---|
Application
A. Western blot analysis of anti-AMPK beta-1 RabMAb (Cat. #AJ1038a), dilution 1:5,000. A-NIH 3T3 B: Hela C: A431 D: PC12
Application
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-AMPK beta-1 RabMAb (Cat. #AJ1038a).| Application Notes | WB:1:5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM226416