Application
Western blot analysis of anti-CAV1 Antibody (N-term) in A2058 cell line lysates (35ug/lane). CAV1(N-term)(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CAV1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CAV1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CAV1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAV1; CAV; Caveolin-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAV |
|---|---|
| Alternative Names | CAV1 CAV Caveolin-1 |
| Molecular Weight(MW) | 20472 Da |
| Function | May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway |
| Tissue Specificity | Expressed in muscle and lung, less so in liver, brain and kidney |
| Cellular Localization | Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein Membrane, caveola; Peripheral membrane protein. Membrane raft Note=Colocalized with DPP4 in membrane rafts. Potential hairpin- like structure in the membrane. Membrane protein of caveolae |
| Entrez Gene | 857 |
|---|
Application
Western blot analysis of anti-CAV1 Antibody (N-term) in A2058 cell line lysates (35ug/lane). CAV1(N-term)(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CAV1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CAV1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Data sheet for OM228788