Application
A. Western blot analysis on HeLa cell lysate using anti-Hsp73 RabMAb (Cat. #AJ1381a).Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Hsp73 RabMAb (Cat. #AJ1381a).Application
C. Immunofluorescent staining of HeLa cells using anti-HSP73 RabMAb (Cat. #AJ1381a).| Product Name | HSP73/HSPA8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPA8, HSC70, HSP73, HSPA10, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1531Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSC70 HSP73 HSPA10 |
|---|---|
| Alternative Names | HSPA8 HSC70 HSP73 HSPA10 Heat shock cognate 71 kDa protein Heat shock 70 kDa protein 8 |
| Molecular Weight(MW) | 70898 Da |
| Function | Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19- CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Melanosome. Nucleus, nucleolus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock |
| Entrez Gene | 3312 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-Hsp73 RabMAb (Cat. #AJ1381a).
Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Hsp73 RabMAb (Cat. #AJ1381a).
Application
C. Immunofluorescent staining of HeLa cells using anti-HSP73 RabMAb (Cat. #AJ1381a).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM235445