Application
Western blot analysis of HSPA8 Antibody (N-term) in Hela cell line lysates (35ug/lane). HSPA8 (arrow) was detected using the purified Pab.Application
Western blot analysis of HSPA8 (arrow) using rabbit polyclonal HSPA8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSPA8 gene.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HSPA8 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of HSPA8 Antibody (N-term)(Cat#AP2872a) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
HSPA8 Antibody (N-term) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HSPA8 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSPA8; HSC70; HSP73; HSPA10; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSC70 HSP73 HSPA10 |
|---|---|
| Alternative Names | HSPA8 HSC70 HSP73 HSPA10 Heat shock cognate 71 kDa protein Heat shock 70 kDa protein 8 |
| Molecular Weight(MW) | 70898 Da |
| Function | Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19- CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Melanosome. Nucleus, nucleolus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock |
| Entrez Gene | 3312 |
|---|
Application
Western blot analysis of HSPA8 Antibody (N-term) in Hela cell line lysates (35ug/lane). HSPA8 (arrow) was detected using the purified Pab.
Application
Western blot analysis of HSPA8 (arrow) using rabbit polyclonal HSPA8 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSPA8 gene.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HSPA8 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of HSPA8 Antibody (N-term)(Cat#AP2872a) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
HSPA8 Antibody (N-term) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Data sheet for OM235495