Application
Flow Cytometry analysis of human Peripheral Blood Lymphocytes (PBL) stained with CD5: FITC antibody (dilution of purified antibody 1 μg/ml) Fig. 1b Dot plotApplication
Flow Cytometry analysis of human Peripheral Blood Lymphocytes (PBL) stained with CD5: FITC antibody (dilution of purified antibody 1 μg/ml) Fig. 1a Histogram - gated on lymphocytes, overlay with isotypic control (mouse IgG2a)| Product Name | CD5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CRIS1] to CD5CD5 is a 67kDa human T-lymphocyte single-chain transmembrane glycoprotein. It is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. It also reacts with a subpopulation of activated B-cells. |
| Immunogen | stimulated human leukocytes |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | CRIS1 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| For FACS: 1 μ g / m l , IP: user optimized, WB: 1 - 2 μ g / m l Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LEU1 T1 |
|---|---|
| Target | CD5 |
| Tissue Specificity | The antibody CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes). |
Application
Flow Cytometry analysis of human Peripheral Blood Lymphocytes (PBL) stained with CD5: FITC antibody (dilution of purified antibody 1 μg/ml) Fig. 1b Dot plot
Application
Flow Cytometry analysis of human Peripheral Blood Lymphocytes (PBL) stained with CD5: FITC antibody (dilution of purified antibody 1 μg/ml) Fig. 1a Histogram - gated on lymphocytes, overlay with isotypic control (mouse IgG2a)| Positive Control | Peripheral Blood Lymphocytes (PBL), Jurkat human leukemia T-cell line, HPB human leukemia T-cell line, MOLT-4 human leukemia T-cell line |
|---|---|
| Application Notes | For FACS: 1 μ g / m l , IP: user optimized, WB: 1 - 2 μ g / m l Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.09% sodium azide |
Data sheet for OM245588