Application
Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CDH1 using rabbit polyclonal CDH1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2).Application
Western blot analysis of CDH1 Antibody in 293 cell line lysates (35ug/lane)| Product Name | E Cadherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO |
| Product description | CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.1) Mansouri,A., Differentiation 38 (1), 67-71 (1988) Knudsen,K.A. J. Cell Biol. 118 (3), 671-679 (1992) Hsu,Y.M., Cancer Res. 67 (22), 11064-11073 (2007) |
| Immunogen | This E Cadherin (CDH1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CDH1 |
|---|---|
| Alternative Names | Cadherin-1 CAM 120/80 Epithelial cadherin E-cadherin Uvomorulin CD324 E-Cad/CTF1 E-Cad/CTF2 E-Cad/CTF3 CDH1 CDHE UVO |
| Molecular Weight(MW) | 97 kDa |
Application
Flow cytometric analysis of NCI-H292 cells using E Cadherin (CDH1) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CDH1 using rabbit polyclonal CDH1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2).
Application
Western blot analysis of CDH1 Antibody in 293 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM277500