Application
Immunohistochemistry staining of HMGA1 in placenta formlin-fixed paraffin-embedded) tissue using HMGA1 Antibody.| Product Name | HMGA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HMGA1, High mobility group protein A1, High mobility group protein R, HMG-R, Hmg-i, HMGIY, High mobility group AT-hook 1, HMG-I(Y), HMGA1A |
| Product description | HMGA1, an 11 kDa basic, DNA-binding non-histone nuclear protein, is a key member of HMGA protein family. It consists of a highly conserved AT-hook DNA-binding domain that mediates binding to AT-rich sequences in the minor groove of chromosomal DNA. It functions as architectural chromatin-binding transcription factor altering the conformation of DNA by modulating nuclear protein-DNA complexes. It is involved in many cellular processes including growth regulation, viral induction of beta-IFN gene and regulation of inducible gene transcription. It also physically interacts with transcription factors such as AP-1, Sp-1 and CAAT/enhancer binding protein-beta to modulate their ability to activate gene expression. It functions as a direct transcriptional target of c-Jun and c-Myc and is maximally expressed during embryonic development. Pathological role of HMGA1 include integration of retroviruses into chromosomes and enhancing metastasis of tumor cells. |
| Immunogen | A synthetic peptide (DGTEKRGRGRPRKQ) corresponding to amino acids 19-32 of human HMGA1, GenBank: XP_001719440.2. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Horse, Rabb ... |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| HMGA1 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.: |
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| Species Reactivity | BatDogGibbonGorillaHamsterHorseHumanMonkeyMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMGA1 |
|---|---|
| Alternative Names | HMGA1 High mobility group protein A1 High mobility group protein R HMG-R Hmg-i HMGIY High mobility group AT-hook 1 HMG-I(Y) HMGA1A |
| Tissue Specificity | The amino acid sequence used as immunogen is 100% homologous in human, dog, mouse, rat, pig, 92% homologous in bovine and 84% homologous in chicken. |
Application
Immunohistochemistry staining of HMGA1 in placenta formlin-fixed paraffin-embedded) tissue using HMGA1 Antibody.| Application Notes | HMGA1 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | HMGA1 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide. |
Data sheet for OM281075