Application
Immunocytochemistry of IRE1p in A20 cells with IRE1p antibody at 1 μg/mL.Application
Immunofluorescence of IRE1p in A20 cells with IRE1p antibody at 10 μg/mL.Application
Western blot analysis of IRE1p in A-20 cell lysate with IRE1p antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | IRE1p Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRE1p Antibody: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.1) Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr.1994; 4:1-18. |
| Immunogen | IRE1p antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human IRE1p. The immunogen is located within amino acids 150 - 200 of IRE1p. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IRE1p antibody can be used for the detection of IRE1p by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERN1 |
|---|---|
| Alternative Names | IRE1p Antibody: IRE1 IRE1P IRE1a hIRE1p IRE1 Endoplasmic reticulum-to-nucleus signaling 1 |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
Application
Immunocytochemistry of IRE1p in A20 cells with IRE1p antibody at 1 μg/mL.
Application
Immunofluorescence of IRE1p in A20 cells with IRE1p antibody at 10 μg/mL.
Application
Western blot analysis of IRE1p in A-20 cell lysate with IRE1p antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | IRE1p antibody can be used for the detection of IRE1p by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRE1p antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRE1p Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM282289