WB
Western blot analysis using ERN1 antibody against HUVEC(1) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ERN1 antibody with DAB staining.ICC/IF
Immunofluorescence analysis of CAL-27 cells using ERN1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Product Name | Anti-ERN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ERN1 |
|---|---|
| Gene Synonyms | IRE1 IRE1P IRE1a hIRE1p |
| Gene Full Name | endoplasmic reticulum to nucleus signaling 1 |
| Gene Summary | This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. |
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Endoplasmic reticulum membrane |
WB
Western blot analysis using ERN1 antibody against HUVEC(1) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ERN1 antibody with DAB staining.
ICC/IF
Immunofluorescence analysis of CAL-27 cells using ERN1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Application Notes | WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM642456