TGF beta(transforming growth factor beta), with 390-amino acid protein (about 43 kDa), is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. In mammals, three isoforms of TGFbeta, that is, beta1, beta2,and beta3,are known. TGF beta is one of numerous inhibitory factors produced by cancer cells that regulate antitumor immunity. TGF beta1 takes part in the local response in the course of primary lung cancer and TGFbeta1 is thought to be implicated in breast cancer progression.TGFbeta1 also plays a critical role in the downregulation of microglial responses minimizing brain inflammation and thus avoiding exacerbation of brain damage.1) Delphine Boche, Colm Cunningham, Fabian Docagne. Neurobiol Dis. 2006 Feb 27
Immunogen
Ni-NTA purified truncated recombinant TGFbeta1 expressed in E. Coli strain BL21 (DE3).
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Host Species
Mouse
Tested Applications
ELISAWB
Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Western blot analysis using TGF beta1 monoclonal antibody against truncated TGF beta1 recombinant protein.
Application Notes
Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Additional Information
Form
Liquid
Storage Instructions
TGF beta1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.