IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NR3C1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NR3C1 antibody with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using NR3C1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Product Name | Anti-NR3C1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC |
| IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NR3C1 |
|---|---|
| Gene Synonyms | GR GCR GRL GCCR GCRST |
| Gene Full Name | nuclear receptor subfamily 3 group C member 1 |
| Gene Summary | This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011] |
| Molecular Weight(MW) | 86KD |
| Cellular Localization | Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus |
IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NR3C1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NR3C1 antibody with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using NR3C1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Application Notes | IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM642533