WB
Western blot analysis of CD52 on different lysates with Rabbit anti-CD52 antibody at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate, Lane 2: Mouse thymus tissue lysate, Lane 3: Mouse liver tissue lysate (negative), Lane 4: Mouse kidney tissue lysate (negative), Lane 5: Mouse brain tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.FC
Flow cytometry analysis of mouse spleen cells labelling CD52.| Product Name | CD52 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:1000 FC:1:1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD52 |
|---|---|
| Gene Synonyms | B7 MB7 CLS1 B7-Ag CAMPATH-1 |
| Gene Full Name | CD52 antigen |
| Gene Summary | Acts upstream of or within response to bacterium. Located in sperm midpiece. Is expressed in embryo mesenchyme and thymus primordium. Orthologous to human CD52 (CD52 molecule). [provided by Alliance of Genome Resources, Feb 2025] |
| Molecular Weight(MW) | 8kDa(Observed band size: 16kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD52 on different lysates with Rabbit anti-CD52 antibody at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate, Lane 2: Mouse thymus tissue lysate, Lane 3: Mouse liver tissue lysate (negative), Lane 4: Mouse kidney tissue lysate (negative), Lane 5: Mouse brain tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
FC
Flow cytometry analysis of mouse spleen cells labelling CD52.| Application Notes | WB:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Data sheet for OM643953