WB
Western Blot using anti-CD52 antibody. Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of anti-CD52 antibody at 1.5 µg/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. anti-CD52 antibody successfully detected CD52 in mouse spleen samples.| Product Name | CD52 Rat Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human peripheral mononuclear cells enriched for T-cells |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Rat |
| Tested Applications | WB |
| WB:1.5ug/ml |
|
| Species Reactivity | HumanMonkeyMouse |
| Purification | Affinity purified |
| Gene Symbol | CD52 |
|---|---|
| Gene Synonyms | B7 |
| Gene Full Name | CD52 molecule |
| Gene Summary | Predicted to be involved in positive regulation of cytosolic calcium ion concentration. Predicted to act upstream of or within response to bacterium. Predicted to be located in plasma membrane and side of membrane. Predicted to be active in sperm midpiece. Orthologous to human CD52 (CD52 molecule). [provided by Alliance of Genome Resources, Feb 2025] |
| Molecular Weight(MW) | 11/25kDa |
| Cellular Localization | Cell membrane. |
WB
Western Blot using anti-CD52 antibody. Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of anti-CD52 antibody at 1.5 µg/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. anti-CD52 antibody successfully detected CD52 in mouse spleen samples.| Application Notes | WB:1.5ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles. |
| Storage Buffer | PBS with 0.02% Proclin 300. |
Data sheet for OM643955