WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-c-Myc antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A172, Lane 2: MCF7.IHC
Human tonsil was stained with Anti-c-Myc rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,c-Myc Antibody(red) was diluted at 1:200(4° overnight). CYCS Monoclonal Antibody(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Product Name | c-Myc Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MYC |
|---|---|
| Gene Synonyms | MRTL MYCC c-Myc bHLHe39 |
| Gene Full Name | MYC proto-oncogene, bHLH transcription factor |
| Gene Summary | This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 48kDa(Calculated), 60kDa(Observed) |
| Cellular Localization | Nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-c-Myc antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A172, Lane 2: MCF7.
IHC
Human tonsil was stained with Anti-c-Myc rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,c-Myc Antibody(red) was diluted at 1:200(4° overnight). CYCS Monoclonal Antibody(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Data sheet for OM644180