Application
Western blot of rat testes lysate showing specific immunolabeling of the ~58k Fto protein using Fatso antibody [5-2H10] ( 82693).| Product Name | Fatso antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5-2H10] to FatsoDioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 5-2H10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | FTO KIAA1752 MGC5149 |
|---|---|
| Target | Fatso |
| Molecular Weight(MW) | 58 (note) |
Application
Western blot of rat testes lysate showing specific immunolabeling of the ~58k Fto protein using Fatso antibody [5-2H10] ( 82693).| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
Data sheet for OM252693