WB
All lanes use the Antibody at 1:2000 dilution for 1 hour at room temperature.WB
Western blot analysis of FTO expression in HEK293 cell lysate.ICC/IF
Immunofluorescent analysis using the Antibody at 1:50 dilution.| Product Name | Rabbit Monoclonal to FTO |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human FTO |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:2000 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FTO |
|---|---|
| Gene Synonyms | GDFD IFEX9 ALKBH9 BMIQ14 |
| Gene Full Name | FTO alpha-ketoglutarate dependent dioxygenase |
| Gene Summary | This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011] |
| Molecular Weight(MW) | 58kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
All lanes use the Antibody at 1:2000 dilution for 1 hour at room temperature.
WB
Western blot analysis of FTO expression in HEK293 cell lysate.
ICC/IF
Immunofluorescent analysis using the Antibody at 1:50 dilution.| Application Notes | WB:1:1000-1:2000 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Data sheet for OM643960