WB
Gel: 8%SDS-PAGE, Lysate: 35μg, Lane 1-6: Jurkat, Hela, HepG2, 293T, SH-SY5Y and NCI-H460 cell lysates, Primary antibody: FTO Antibody at dilution 1/500, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 15 minutes.IHC
The image is immunohistochemistry of paraffin-embedded Human pancreas tissue using FTO Antibodyat dilution 1/500. (Original magnification: ×200).| Product Name | Anti-FTO mouse monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Fusion protein of human FTO |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:200-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FTO |
|---|---|
| Gene Synonyms | GDFD IFEX9 ALKBH9 BMIQ14 |
| Gene Full Name | FTO alpha-ketoglutarate dependent dioxygenase |
| Gene Summary | This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011] |
| Molecular Weight(MW) | 58kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Gel: 8%SDS-PAGE, Lysate: 35μg, Lane 1-6: Jurkat, Hela, HepG2, 293T, SH-SY5Y and NCI-H460 cell lysates, Primary antibody: FTO Antibody at dilution 1/500, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 15 minutes.
IHC
The image is immunohistochemistry of paraffin-embedded Human pancreas tissue using FTO Antibodyat dilution 1/500. (Original magnification: ×200).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Data sheet for OM643961