Application
Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 μg/mL.Application
Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2.5 μg/mL.Application
Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/mL.| Product Name | FTO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FTO Antibody: ALKBH9, KIAA1752, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein |
| Product description | FTO Antibody: Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.1) Scuteri A, Sanna S, Chen W-M, et al. Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits. PloS Genet.2007; 3:e115. |
| Immunogen | FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO. The immunogen is located within the first 50 amino acids of FTO. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FTO |
|---|---|
| Alternative Names | FTO Antibody: ALKBH9 KIAA1752 Alpha-ketoglutarate-dependent dioxygenase FTO Fat mass and obesity-associated protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (100%) |
Application
Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 μg/mL.
Application
Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2.5 μg/mL.
Application
Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1317 - Human Uterus Tissue Lysate |
|---|---|
| Application Notes | FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FTO antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FTO Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM279256