Immunohistochemical
Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution :1:300Secondary Antibody : Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution :1:200Color/Signal Descriptions :CPT1A: Green DAPI: BlueGene Name :CPT1ASubmitted by :Dr. Pankaj Singh, UNMC, Omaha, NEImmunoblot
1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CPT1ASubmitted by: Dr. Pankaj Singh, UNMC, Omaha, NEWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Product Name | Rabbit anti-CPT1A polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CPT1, CPT1-L, L-CPT1 |
| Immunogen | The immunogen for anti-CPT1A antibody: synthetic peptide directed towards the middle region of human CPT1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CPT1A |
|---|---|
| Gene Synonyms | CPT1 CPT1-L L-CPT1 |
| Gene Full Name | Carnitine palmitoyltransferase 1A (liver) |
| Gene Summary | The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | CPT1 CPT1-L L-CPT1 |
| Molecular Weight(MW) | 86kDa |
| Sequence | 756 amino acids |
| Entrez Gene | 1374 |
|---|---|
| SwissProt ID | P50416 |
| Protein Accession | NP_001027017 |
Immunohistochemical
Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution :1:300Secondary Antibody : Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution :1:200Color/Signal Descriptions :CPT1A: Green DAPI: BlueGene Name :CPT1ASubmitted by :Dr. Pankaj Singh, UNMC, Omaha, NE
Immunoblot
1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CPT1ASubmitted by: Dr. Pankaj Singh, UNMC, Omaha, NE
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM111925