Application
Left image is paraformaldehyde-fixed and paraffin-embedded cow lactating with CPT1A Pab , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining, right image is contrast, did not add the antibody. This data was kindly offered by Hideaki Hayashi, University of Bern, Switzerland.Application
Western blot analysis of anti-CPT1A Pab in Y79 cell line lysate (35ug/lane).| Product Name | CPT1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPTI-L, Carnitine palmitoyltransferase 1A, CPT1A, CPT1 |
| Product description | The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.1) Rasmussen, B.B., et al., J. Clin. Invest. 110(11):1687-1693 (2002). |
| Immunogen | This CPT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 606-636 amino acids from the C-terminal region of human CPT1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | BovineHuman |
| Concentration | 1mg/ml |
| Gene Symbol | CPT1A |
|---|---|
| Alternative Names | Carnitine O-palmitoyltransferase 1 liver isoform CPT1-L Carnitine O-palmitoyltransferase I liver isoform CPT I CPTI-L Carnitine palmitoyltransferase 1A CPT1A CPT1 |
| Molecular Weight(MW) | 88 kDa |
Application
Left image is paraformaldehyde-fixed and paraffin-embedded cow lactating with CPT1A Pab , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining, right image is contrast, did not add the antibody. This data was kindly offered by Hideaki Hayashi, University of Bern, Switzerland.
Application
Western blot analysis of anti-CPT1A Pab in Y79 cell line lysate (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM276057