Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | CPT1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.1) Gessner, B.D., et al. Pediatrics 126(5):945-951(2010) |
| Immunogen | This CPT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-756 amino acids from the C-terminal region of human CPT1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CPT1A |
|---|---|
| Alternative Names | Carnitine O-palmitoyltransferase 1 liver isoform CPT1-L Carnitine O-palmitoyltransferase I liver isoform CPT I CPTI-L Carnitine palmitoyltransferase 1A CPT1A CPT1 |
| Molecular Weight(MW) | 88 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM276054