Application
Antibody used in IHC on Human Capan1 at 1:300.Application
Antibody used in WB on Human capan1, HPAF at: 1:1000 (Lane 1. 45ug capan1 cell lysate Lane 2. 45 ug HPAF cell lysate).Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | CPT1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.1) Roomets, E., (2008) Invest. Ophthalmol. Vis. Sci. 49 (4), 1660-1664. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CPT1A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CPT1A antibody can be used for detection of CPT1A by ELISA at 1:312500. CPT1A antibody can be used for detection of CPT1A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CPT1A |
|---|---|
| Alternative Names | CPT1A CPT1 CPT1-L L-CPT1 |
| Molecular Weight(MW) | 86 kDa |
| Entrez Gene | 1374 |
|---|---|
| Protein Accession | NP_001027017 |
Application
Antibody used in IHC on Human Capan1 at 1:300.
Application
Antibody used in WB on Human capan1, HPAF at: 1:1000 (Lane 1. 45ug capan1 cell lysate Lane 2. 45 ug HPAF cell lysate).
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | CPT1A antibody can be used for detection of CPT1A by ELISA at 1:312500. CPT1A antibody can be used for detection of CPT1A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CPT1A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM276055