Immunofluorescence
Sample Type: Mouse Brain Slices Red: primaryBlue: DAPIPrimary Dilution: 1:400 Secondary Antibody: Anti-Rabbit IgG Alexa 594Secondary Dilution: 1:400 Image Submitted By: Adahir Labrador-Garrido and Cintia Roodveldt University of SevilleWestern blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: Hela Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: HIRIP3 Sample Tissue: 293T Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: WT1 Sample Tissue: 721_B Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: NOP56 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: RHOT1 Sample Tissue: Jurkat Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysateWestern blot
HSPA8 antibody - N-terminal region validated by WB using Rat Brain lysate at 1:1,000.| Product Name | Rabbit anti-HSPA8 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, MGC131511, MGC29929, NIP71 |
| Immunogen | The immunogen for anti-HSPA8 antibody: synthetic peptide directed towards the N terminal of human HSPA8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPA8 |
|---|---|
| Gene Synonyms | HSC54 HSC70 HSC71 HSP71 HSP73 HSPA10 LAP1 MGC131511 MGC29929 NIP71 |
| Gene Full Name | Heat shock 70kDa protein 8 |
| Gene Summary | HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. The protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. |
| Alternative Names | HSC54 HSC70 HSC71 HSP71 HSP73 HSPA10 LAP1 MGC131511 MGC29929 NIP71 |
| Molecular Weight(MW) | 71kDa |
| Sequence | 646 amino acids |
Immunofluorescence
Sample Type: Mouse Brain Slices Red: primaryBlue: DAPIPrimary Dilution: 1:400 Secondary Antibody: Anti-Rabbit IgG Alexa 594Secondary Dilution: 1:400 Image Submitted By: Adahir Labrador-Garrido and Cintia Roodveldt University of Seville
Western blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: Hela Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: HIRIP3 Sample Tissue: 293T Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: WT1 Sample Tissue: 721_B Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: NOP56 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: RHOT1 Sample Tissue: Jurkat Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate
Western blot
HSPA8 antibody - N-terminal region validated by WB using Rat Brain lysate at 1:1,000.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM113262