Immunohistochemistry
Anti-HSPA8 / HSC70 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibodyconcentration 5 ug/ml.Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: COLO205 cell lysateWestern blot
Titer: 1:1000Positive Control: Rat Brain Lysate| Product Name | Rabbit anti-HSPA8 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, MGC131511, MGC29929, NIP71 |
| Immunogen | The immunogen for anti-HSPA8 antibody: synthetic peptide directed towards the N terminal of human HSPA8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogMouseRabbitZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPA8 |
|---|---|
| Gene Synonyms | HSC54 HSC70 HSC71 HSP71 HSP73 HSPA10 LAP1 MGC131511 MGC29929 NIP71 |
| Gene Full Name | Heat shock 70kDa protein 8 |
| Gene Summary | HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. The protein binds to nascent polypeptides |
| Alternative Names | HSC54 HSC70 HSC71 HSP71 HSP73 HSPA10 LAP1 MGC131511 MGC29929 NIP71 |
| Molecular Weight(MW) | 71kDa |
| Sequence | 646 amino acids |
Immunohistochemistry
Anti-HSPA8 / HSC70 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibodyconcentration 5 ug/ml.
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: COLO205 cell lysate
Western blot
Titer: 1:1000Positive Control: Rat Brain Lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM113265