WB
Western blot analysis using Parkin antibody against PANC-1 (1),MCF-7 (2), COS-7 (3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded pancreatic tissues using Parkin antibody with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using Parkin antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Product Name | Anti-Parkin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKN |
|---|---|
| Gene Synonyms | HSPA8 HSP7C LAP1 HSC54 HSC70 HSC71 HSP71 HSP73 LAP-1 NIP71 HEL-33 HSPA10 HEL-S-72p |
| Gene Full Name | parkin RBR E3 ubiquitin protein ligase |
| Gene Summary | The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 52 kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus |
WB
Western blot analysis using Parkin antibody against PANC-1 (1),MCF-7 (2), COS-7 (3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded pancreatic tissues using Parkin antibody with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using Parkin antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.| Application Notes | WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM642080